An Easy and Simple Approach for Biosynthesis of Selenium Nanoparticles; Characterization, Radiolabeling and Biomedical Application

Elkhouly, Heba Ibrahim; Nasr, Zeinab S.; Hassane, Mohamed K.

doi:10.21608/ejchem.2025.338679.10892

An Easy and Simple Approach for Biosynthesis of Selenium Nanoparticles; Characterization, Radiolabeling and Biomedical Application

Articles in Press

Document Type : Original Article

Authors

¹ Yossuf Abbas St.

² Labeled Compounds Department., Hot Labs Center, Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt.

³ Radioisotopes Production Facility (RPF), Egyptian Second Research Reactor (ETRR‑2), Atomic Energy Authority, P.O. Box 13759, Cairo, Egypt

10.21608/ejchem.2025.338679.10892

Abstract

Nanomaterials biogenic approaches of synthesis exceed chemical ways in simplicity, toxicity and generation of homogeneous colloidals. Herein, a rare approach for biosynthesis of SeNPs is described where a rapid myogenic method was applied. The endophytic fungus isolate, Asperigillus terrus H1 with accession no. PQ550684, that isolated for the first time from Rumex thyrsiflorus was used for the bio-reduction of sodium selenite contained in the growth medium to produce selenium nanoparticles. Change in color of the culture medium to orange-red signifies the formation of nano-selenium. DLS, TEM and ICP-OES techniques demonstrated the formation of monodispersive amorphous selenium nanoparticles with average hydrodynamic size of 55.76 nm with -25.6 particles charge. The synthesized SeNPs were checked for their in-vitro cytotoxicity against normal human lung fibroblast cells (MRC-5) and human lung adenocarcinoma cell line (A549) which giving IC50 of 485.16±13.71 and 196.25± 4.23 µg/ml respectively. Their ability to be applied in biomedical applications was studied in-vivo through labeling them with 99mTc and tracing their distribution in normal and tumor-bearing mice. The produced mycogenic SeNPs showed excellent physical and biological characteristics that could make them a promising nanoplatform for tumor molecular imaging.

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